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Reading HDF5 Files In The Cloud16 days ago
Public S3 Buckets | Private S3 Buckets | Session Info
Supported Zarr features in Rarr16 days ago
Zarr version | Reading and Writing | Stores | Data Types | Codecs | Compression codecs | Other codecs | Optional fields or features
Design principles for the Rarr package2 months ago
Guiding principles | Scope | Zarr version | Functional programming and API design
Introduction: microarray quality assessment with arrayQualityMetrics3 months ago
Introduction | Basic use | Affymetrix data - before preprocessing | Affymetrix data - after preprocessing | ExpressionSet and ExpressionSetIllumina | Two colour arrays, NChannelSet, RGList, MAList | Loading data from ArrayExpress | Making the report more informative by adding a factor of interest | Extended use | Spatial layout of the array | Mapping of the reporters | RNA quality | Session Info | References
Advanced topics: Customizing arrayQualityMetrics reports and programmatic processing of the output3 months ago
Introduction | Data preparation | Module generating functions | Outlier detection | Rendering the report | Session Info
Working with Zarr arrays in R3 months ago
Introduction | Limitations with Rarr | Example data | Quick start guide | Additional details | Working with Zarr metadata | Using credentials to access S3 buckets | Creating an S3 client | Writing subsets of data | Creating an "empty" array | Updating a subset of an existing array | Appendix | Session info
Linking to Rhdf5lib3 months ago
Motivation | Usage | Link to the library | Locating the library headers | Configuration arguments for non-standard system setups | Funding | Session info
rhdf5 Practical Tips4 months ago
Introduction | Reading subsets of data | Using the index argument | Using hyperslab selections | Irregular selections | Using hyperslab selection tools | Slowdown when selecting unions of hyperslabs | Summary | Writing in parallel | Example data | Serial writing of datasets | Parallel writing of datasets | Session info
rhdf5 - HDF5 interface for R7 months ago
Introduction | High level R-HDF5 functions | Creating an HDF5 file and group hierarchy | Writing and reading objects | Writing and reading objects with file, group and dataset handles | Saving multiple objects to an HDF5 file (h5save) | List the content of an HDF5 file | Dump the content of an HDF5 file | Reading HDF5 files with external software | Removing content from an HDF5 file | 64-bit integers | Low level HDF5 functions | Creating an HDF5 file and a group hierarchy | Writing data to an HDF5 file | Session Info
Using a BioMart other than Ensembl9 months ago
Introduction | Wormbase | Phytozome | Version 12 | Version 13 | Session Info
Accessing Ensembl annotation with biomaRt9 months ago
Introduction | Selecting an Ensembl BioMart database and dataset | Step1: Identifying the database you need | Step 2: Choosing a dataset | Ensembl mirror sites | Using archived versions of Ensembl | Using Ensembl Genomes | How to build a biomaRt query | Searching for filters and attributes | Using predefined filter values | Finding out more information on filters | filterType | Attribute Pages | Using select() | Result Caching | biomaRt helper functions | exportFASTA | Examples of biomaRt queries | Annotate a set of Affymetrix identifiers with HUGO symbol and chromosomal locations of corresponding genes | Annotate a set of EntrezGene identifiers with GO annotation | Retrieve all HUGO gene symbols of genes that are located on chromosomes 17,20 or Y, and are associated with specific GO terms | Annotate set of idenfiers with INTERPRO protein domain identifiers | Select all Affymetrix identifiers on the hgu133plus2 chip and Ensembl gene identifiers for genes located on chromosome 16 between basepair 1100000 and 1250000. | Retrieve all EntrezGene identifiers and HUGO gene symbols of genes which have a "MAP kinase activity" GO term associated with it. | Given a set of EntrezGene identifiers, retrieve 100bp upstream promoter sequences | Retrieve all 5' UTR sequences of all genes that are located on chromosome 3 between the positions 185,514,033 and 185,535,839 | Retrieve protein sequences for a given list of EntrezGene identifiers | Retrieve known SNPs located on the human chromosome 8 between positions 148350 and 148400 | Given the human gene TP53, retrieve the human chromosomal location of this gene and also retrieve the chromosomal location and RefSeq id of its homolog in mouse. | Connection troubleshooting | r BiocStyle::Biocpkg("biomaRt") specific solutions | Global connection settings | Error: "SSL certificate problem" | Error: "sslv3 alert handshake failure" | Session Info
beadarray2 years ago
beadarray: R classes and methods for Illumina bead-based data | Introduction | Companion packages to beadarray | Citing beadarray | Getting help
Analysis of bead-summary data2 years ago
feature and pheno data# | Subsetting the data | Exploratory analysis using boxplots | A note about ggplot2 | Other exploratory analysis | Normalisation | Filtering | Differential expression | Automating the DE analysis | Output as GRanges | Visualisation options | Creating a GEO submission file | Analysing data from GEO | Reading bead summary data into beadarray | Reading IDAT files | Citing beadarray | Asking for help on beadarray
Analysis of Bead-level Data using beadarray2 years ago
beadarray: R classes and methods for Illumina bead-based data | Analysis of Bead-level Data using beadarray} | Citing beadarray | Asking for help on beadarray | Reading bead-level data into beadarray | File formats | A note for those with iScan data | Array annotation | The beadLevelData class | Scan Metrics | Transformation Functions | BASH | Summarization
Image Analysis with beadarray2 years ago
Introduction | Reading bead-level data into beadarray | Standard Illumina Image Processing} | Alternative Methods | Parallel Processing | References
HDF5 Compression Filters2 years ago
Motivation | Usage | With rhdf5 | Writing data | Reading data | With external applications | h5dump example | Compiling the compression libraries | Session info
Reading FIT files3 years ago
Introduction | Installing and loading the library | Example fit files | Reading files | Working with the data | Records - GPS, speed, altitude, etc | Extracting common data types | Accessing any data type | Example use cases | Plotting a route | Comparing heart rate measurments between devices | More details | Data types and units | Session Info
Converting Rmarkdown to F1000Research LaTeX Format8 years ago
Introduction | Citing this work | Package installation | Creating a new workflow document | Using RStudio and our template | Working outside RStudio | Converting to LaTeX and PDF | Article upload | R session information | Acknowledgements and funding
BeadDataPackR Vignette8 years ago
BeadDataPackR Vignette8 years ago